Analisis cemaran mikrobiologis pangan asal ternak pada jajanan anak sekolah dasar
Microbiological analysis contamination of children’s snacks elementary school
DOI:
https://doi.org/10.46549/jipvet.v14i2.477Keywords:
Contamination, E. coli, Microbes, Salmonella sp, SnacksAbstract
Abstract
This study examined the microbiological contamination of street food that consumed by children at primary. The study, specifically focused on the detection of total microorganisms such as Escherichia coli, and Salmonella sp. Microbiological analyses is one way to determine the level of hygiene of snack foods. Presence of microbes determined by using Total Plate Count (TPC), detection test was applied to detect E.coli and Salmonella sp test applied detect present of Salmonella sp. Quantitatif and qualitatif methods have been sed with sampel snacks collection were obtained street food at primary schools. TPC test results showed that 6 samples of snacks have been contaminated with microbes with the threshold of the maximum limit. Those snacks, namely G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5 cfu/gr, L2(egg meat ball) 2.3 x 10-5 cfu/gr, M1 (egg roll) 3.8 x 10-5 cfu/gr, N (meatball) 1.1 x 10-5 cfu/gr and Q1 (batagor) 1.1 x 10-5 cfu/gr. Results Salmonella sp test indicated that there were 9 samples positively contaminated with salmonella sp bacteria. Those are D2 (chicken sausage), E1 (egg roll), E2 (chicken meat ball), F2 (chicken meat ball), G1 (batagor), G2 (fried sausage), N (meatball), P1 (nuggets), P2 (chicken meat ball). Escherchia coli detection test results all samples were declared negative. There were 6 school locations (G, K, L, M, N, Q) different snack samples contaminated with microbes, 6 school locations (D, E, F, G ,N, P) snack samples were contaminated with Salmonella sp. Those snacks were declared not recommended for consumption.
Keywords: Contamination; E. coli; Microbes; Salmonella sp; Snacks
Abstrak
Penelitian ini meneliti kontaminasi mikrobiologi pada jajanan yang ditujukan untuk anak-anak sekolah dasar di distrik Manokwari Barat, dengan fokus khusus pada deteksi mikroorganisme total, E. coli, dan Salmonella sp. Salah satu cara untuk mengetahui tingkat higienitas dari pangan jajanan adalah dengan analisis mikrobiologi berupa uji keberadaan mikroba dengan Total Plate Count (TPC), uji deteksi E.coli dan uji Salmonella sp. Sampel jajanan hasil olahan ternak diperoleh dari jajanan yang ada di Sekolah Dasar di wilayah distrik Manokwari Barat. Penelitian ini menggunakan gabungan metode kualitatif dan kuantitatif dengan menggunakan teknik pengambilan sampel yaitu purposive sampling. Hasil uji TPC pada jajanan pangan hasil ternak di Sekolah Dasar distrik Manokwari Barat terdapat 6 sampel yang berada diambang batas maksimum yaitu sampel G1 (batagor) 2.6 x 10-5 cfu/gr, K1 (batagor) 1.3 x 10-5cfu/gr L2 (pentol telur) 2.3 x 10-5 cfu/gr. M1 (telur gulung) 3.8 x 10-5 cfu/gr, N (pentol bakso) 1.1 x 10-5 cfu/gr dan Q1 (batagor) 1.1 x 10-5 cfu/gr. Hasil pengujian deteksi Salmonella sp terdapat 9 sampel yang positif tercemar bakteri Salmonella sp. D2 (sosis ayam), E1 (telur gulung), E2 (pentol ayam), F2 (pentol ayam), G1 (batagor), G2 (sosis goreng), N (pentol bakso), P1 (nugget), P2 (pentol ayam) sedangkan untuk hasil uji deteksi E. coli semua sampel dinyatakan negatif. Dari hasil penelitian ini dinyatakan bahwa ada 6 lokasi (G, K, L, M, N, Q) telah terkontaminasi mikroba diambang batas dan tidak layak untuk dikonsumsi dan 6 lokasi (D, E, F, G, N, P) telah terkontaminasi Salmonella sp dan dinyatakan tidak layak untuk dikonsumsi.
Kata kunci: E. coli; Mikroba; Pangan jajanan; Salmonella sp; Total plate count
Downloads
References
Abebe, E., Gugsa, G., and Ahmed, M. 2020. Review on major food-borne zoonotic bacterial pathogens. Journal of Tropical Medicine; 1-19.
https://doi.org/10.1155/2020/4674235
Alam, S.T., Howard M., Fatema K., Haque K., F. M. 2015. Antibiogram of preprocessed raw chicken meat from different supershops of Dhaka City. Bangladesh. J. All Health Sci, 83; 1093-1098.
Amri, F., Sayuti, A. dan Darniati. 2017. Isolasi dan identifikasi bakteri enterik pada feses gajah sumatera (Elephas maximus sumatranus) di pusat konservasi gajah (PKG) Saree Aceh Besar. JIMVET, 01(3); 305-315.
Andriyani, A., Gunawan, I.M., & Susilo, J.E. 2009. Efektivitas penurunan jumlah angka kuman alat makan dan efisiensi biaya yang digunakan pada metode pencucian alat makan di Rumah Sakit kota Surakarta. Jurnal Gizi Klinik Indonesia, 6(1).
https://doi.org/10.22146/ijcn.17687
Apriani, L., R. dan R. K. 2019. Deteksi bakteri Salmonella dan Shigella pada makanan burger di Sungai Raya Dalam Pontianak. Jurnal Protobiont, 8(3); 53-57.
https://doi.org/10.26418/protobiont.v8i3.36836
Budiarso, Tri Y., Maria J. X. B. 2009. Deteksi cemaran Salmonella pada daging ayam yang dijual di pasar tradisional di wilayah kota Yogyakarta. Prosiding Seminar Nasional Penelitian Pendidikan dan Penerapan MIPA. Yogyakarta : UNY
Dhafin, A. A. 2017. Analisis Cemaran Bakteri Coliform Escherichia Coli pada Bubur Bayi Home Industry di Kota Malang dengan Metode TPC dan MPN. Universitas Islam Negeri Maulana Malik Ibrahim.
Fung, F., Wang, HS., and Menon, S. 2018. Food safety in the 21st century. Biomedical Journal, 41(2); 88-95.
https://doi.org/10.1016/j.bj.2018.03.003
Hariri, S. 2022. Detection of Escherichia Coli in Food Samples Using Culture and Polymerase Chain Reaction Methods. Cureus.
https://doi.org/10.7759/cureus.32808
Hariyadi, P. 2010. Penanganan Kontaminan Pangan dalam Rangka Menjamin Keamanan Pangan. Workshop Pokja Keamanan Pangan Rencana Aksi Nasional Pangan dan Gizi 2011-2015.
Kemal, J. 2014. A review on the public health importance of bovine salmonellosis. Journal of Veterinary Science and Technology, 5(2); 1-10.
Kothari, A., Pruthi, A. dan Chugh, T. D. 2008. The burden of enteric fever. Journal of Infection in Developing Countries, 2(4); 253-259.
https://doi.org/10.3855/jidc.218
Maturin, L., & Peeler, J. T. 2001. Hitungan Lempeng Aerobik | FDA.
Nurmawati, S. Prodjosoewojo, S. Chairunnisa, NH., Djauhari, H., dan Alisjahbana, B. 2019. Faktor risiko penyebab foodborne disease pada siswa SD. Jurnal Sains dan Kesehatan, 4(4); 180- 184.
Okolie, N.P, Omonigbehin, E., Badru, O, A. And Akande, I.S. 2012. Isolation of pathogenic bacteria from some foods sold at selected private school in Akoka area of Yaba Lagos. African Journal of Food Science, 6; 65-69.
https://doi.org/10.5897/AJFS11.187
Sauli, LJ, Danuser C, Wenk, Stark KDC. 2003. Evaluation of the safety assurance level for Salmonella spp. Through out the Food Production Chain in Switzerland. J Food Prot, 66; 1139-1145.
https://doi.org/10.4315/0362-028X-66.7.1139
Siyam, N dan Cahyati, WH. 2018. Peningkatan kapasitas penghuni pondok pesantren dalam pencegahan food borne diseases dengan metode peer education. Visikes Jurnal Kesehatan Masyarakat, 17(2); 136-147.
Standar Nasional Indonesia. 2008. Metode Pengujian Cemaran Mikroba dalam Daging, Telur dan Susu, serta Hasil. Badan Standarisasi Nasional.
Suwandono, A.M., Destri, dan C. S. 2005. Salmonellosis dan Surveillans Demam Tifoid yang Disebabkan Salmonella di Jakarta Utara. Disampaikan dalam Lokakarya Jejaring Intelijen Pangan. Jakarta. BPOM RI.
Trigunarso, SI. 2020. Hygiene sanitasi dan perilaku penjamah makanan dengan angka kuman pada makanan jajanan di lingkungan sekolah. Jurnal Kesehatan, 11(1).
https://doi.org/10.26630/jk.v11i1.1739
White, D. G. 2001. Salmonella from retail ground meats. Engl. J. Med, 345; 1147-1154.
https://doi.org/10.1056/NEJMoa010315
World Health Organization. 2019. Foodborne Disease. Retrieved from https://www.who.int/healthtopics/foodborne-diseases#tab=tab_1.
Wulandari, S. 2022. Perancangan E-Booklet tentang pangan jajanan anak sekolah (PJAS) berbahaya untuk siswa SD. Jurnal Seni Rupa dan Desain, 25(1).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Noveling Inriani, Sientje Daisy Rumetor, Angelina Novita Tethool
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
License and Copyright Agreement
In submitting the manuscript to the journal, the authors certify that:
- They are authorized by their co-authors to enter into these arrangements.
- The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal. Please also carefully read Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Posting Your Article Policy at https://journal.fapetunipa.ac.id/index.php/JIPVET/publicationethics
- That it is not under consideration for publication elsewhere,
- That its publication has been approved by all the author(s) and by the responsible authorities “tacitly or explicitly“ of the institutes where the work has been carried out.
- They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
- They agree to the following license and copyright agreement.
Copyright
Authors who publish with Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-NC-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Most read articles by the same author(s)
- Angelina Novita Tethool, Gatot Ciptadi, Sri Wahjuningsih, Trinil Susilawati, Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 12 No. 1 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Sonria Aritonang, Sientje Daisy Rumetor, Onesimus Yoku, Pertumbuhan Vegetatif Rumput Raja (Pennisetum purpureophoides) dengan Perlakuan Pupuk Anorganik Dan Organik , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Mei Isnaeni, O R Faidiban, Angelina Novita Tethool, Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam) , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 9 No. 2 (2019): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
- Kiki Inggriani, Angelina Novita Tethool, Sintje Lumatauw, Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Noveling Inriani, Priyo Sambodo, Identifikasi cacing nematoda pada saluran pencernaan babi di Makassar , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 12 No. 3 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Angelina Novita Tethool, Abdul Rahman Ollong, Johan Fredrik Koibur, Pengaruh Sari Buah Merah (Pandanus conoideus Lam) terhadap Abnormalitas Spermatozoa Ayam Kampung , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 11 No. 2 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Iwung Sri Suryaningsih, Sientje Daisy Rumetor, Andoyo Supriyantono, Irba Unggul Warsono, Analisis Faktor-Faktor Produksi Usaha Pengembangan Sapi Potong Di Kabupaten Teluk Bintuni , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 12 No. 1 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
- Angelina Novita Tethool, Raden Iis Arifiantini, Srihadi Agungpriyono, KONSENTRASI DAN MOTILITAS SPERMATOZOA CAUDA EPIDIDIMIS BANDIKUT (Echymipera kalubu) , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 7 No. 1 (2012): JURNAL ILMU PETERNAKAN
- Deny Anjelus Iyai, Mulyadi Mulyadi, Muhammad Jen Wajo, Rosdiana Naibey, Dwi Nur Hayati, Noviyanti Noviyanti, Makarius Bajari, Johan Koibur, Yafed Syufi, Stepanus Pakage, Noveling Inriani, Lamberthus E. Nuhuyanan, Jublyana Purba, Bernadetha W.I. Rahayu, Hendrik Fatem, Isti Widayati, Defining and Valuing the Relationship Pattern of Actors' Involvement on Cattle Farming Systems using Stakeholder Network Analysis in West New Guinea, Indonesia , Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science): Vol. 11 No. 3 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science)
