Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)

Concentration and Motility Spermatozoa of Ayam Kampung (Gallus domesticus) in Ringer Laktat Diluents Adding Red Fruit Oil (Pandanus conoideus Lam)

  • Mei Isnaeni Universitas Papua, Manokwari
  • O R Faidiban Universitas Papua, Manokwari
  • Angelina Novita Tethool Indonesia

Abstract

Red fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3),  ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86%  to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P <0.01) on motility 0.05). The spermatozoa concentration ranged from 1,600x106 cells / ml to 2,200x106 cells/ml. The spermatozoa concentration of treatment P1 and P2 chickens did not show any significant differences (P> 0.05), nor did among treatments P2, P3 and P4.  However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil  up to concentrations of more than 2% tend to reduce sperm motility.


Keywords: Concentration, Motility, Spermatozoa and Red fruit oil

Downloads

Download data is not yet available.

Author Biographies

Mei Isnaeni, Universitas Papua, Manokwari

SubLab Fisiologi dan Reproduksi Ternak. Fakultas Peternakan Universitas Papua.

Jl. Gunung Salju Amban Manokwari Papua Barat 98314

O R Faidiban, Universitas Papua, Manokwari

SubLab Fisiologi dan Reproduksi Ternak. Fakultas Peternakan Universitas Papua.

Jl. Gunung Salju Amban Manokwari Papua Barat 98314

Angelina Novita Tethool, Indonesia

SubLab Fisiologi dan Reproduksi Ternak. Fakultas Peternakan Universitas Papua.

Jl. Gunung Salju Amban Manokwari Papua Barat 98314

References

Al-Daraji, H. J. 2012a. Adding olive oil to rooster semen diluents for improving semen quality and storage ability during liquid. International Science Index, Animal and Veterinary Sciences, 6(10):862-867.
Al-Daraji, H. J. 2012b. Effect of diluent supplementation with different levels of orange juice on semen quality during liquid storage of roosters’ semen. International Journal Veteriner Science, 1(1): 5-9.
Al-Daraji, H. J. 2012c. Red wine as a diluent supplement for counteract the deleterious effects of lipid peroxidation during liquid storage of aged roosters semen. Nature and Science, 10(9):64-70.
Al-Daraji, H. J. 2013. Effect of diluent supplementation with garlic extract on semen quality of cocks during liquid storage. International Journal of Pharma and Bio Scienses, 4(3): 1260 – 1270.
Al-Daraji, H. J. 2014. Effect of diluent supplementation with tomato juice on semen quality and storage ability of roosters semen during liquid storage. International Journal of Biology, Pharmacy and Allied sciences, 1(7):918-926.
Al-Daraji, H. J. 2015. Effects of pomegranate juice as diluent supplementation on sperm quality during liquid storage of chicken semen. International Journal of Chemistry and Pharmaeutical sciences, 2(1):606-611.
Blesbois, E., Grasseau, I. and Hermier, D. 1999. Changes in lipid content of fowl spermatozoa after liquid storage at 2 to 50C. Theriogenology, 52:325–334.
Budi, M. dan Paimin, F. R. 2004. Sari Buah Merah Sebagai Obat Multi Khasiat. Jakarta: Penebar swadaya.
Bramwell, K. R., Marks, H. L. and Howarth, B. 1995. Physiology and reproduction quantitative determination of spermatozoa penetration of the perivitelline layer of the hen's ovum as assessed on oviposited eggs. Poultry Science, 74 : 1875-1883.
Chabory, E., Damon, C., Lenoir, A., Henry-Berger, J., Vernet, P., Cadet, R., et al. 2010. Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity. Journal Animal sciences, 88: 1321–1331.
Funahashi, H. and Sano, T., 2005. Selected antioxidants improve the function of extended boar semen stored at 100C. Theriogenology, 63(6): 1605-1616.
Graham, E. F. 1978. Fundamentals of the Preservation of Spermatozoa. In The Integrity of Frozen Spermatozoa. National Academy of Sciences, Washington D.C. p228.
Gunawan, I., Laksmi, D. N. D. I. dan Trilaksana, I. G. N. B. 2012. Efektivitas penambahan β-karoten dan glutathion pada bahan pengencer terhadap motilitas dan daya hidup spermatozoa pada semen beku sapi. Indonesia Medicus Veterinus, 1(3) : 385 – 393.
Hafez, E. S. E. 2000. Reproduction in Farm Animals. (7 th ed) Kiawah island, south Carolina, USA.
Indrawati, D., Bebas, W., dan Trilaksana, I. G. N. B. 2013. Motilitas dan daya hidup spermatozoa ayam kampung dengan penambahan astaxanthin pada suhu 3– 50C. Indonesia Medicus Veterinus, 2(4) : 445 – 452.
Khaeruddin., Sumantri, C., Darwati, S. dan Arifiantini, R. I. 2015. Penggunaan minyak zaitun ekstrak virgin ke dalam bahan pengencer semen terhadap kualitas spermatozoa ayam lokal. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 3(1) : 46-51.
Long, J. A. and Kramer, M. 2003. Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Sciences, (82) : 1802–1807.
Lubis, T. M. 2011. Motilitas spermatozoa ayam kampung dalam pengencer air kelapa, NaCl fisiologis dan air kelapa-NaCl fisiologis pada 25-29°C. Agripet, 11(2) : 45-50.
Murugesan, S., Matam, N., Kulkarni, R., Bhattacharya, T. K. and Chatterjee, R. N. 2013 Semen quality in white leghorn chicken selected for egg production traits turk. J. Vet. Anim. Sci, 37: 747-749.
Partyka, A., Jerysz, A., Pokorny, P. 2007. Lipid peroxidation in fresh and stored semen of green-legged partridge. Electronic Journal of Polish Agricultural Universities,10(2):08.fromhttp://www.ejpau.media.pl/volume10/issue2/art-08.html
Peters, S. O., Shoyebo, O. D., Ilori, B. M., Ozoje, M. O., Ikeobi, C. O. N. Et al. 2008. Semen quality traits of seven strain of chickens raised in the humid tropics. Int. J. Poult. Sci, 7: 949-953.
Rizal, M. dan Herdis. 2005. Daya hidup spermatozoa epididimis domba garut yang dikriopreservasi menggunakan modifikasi pengencer tris. Hayati, 12(2) : 61-66.
Solihati, N. 2008. Studi terhadap kualitas dan daya tahan hidup spermatozoa cauda epididimis domba garut menggunakan berbagai jenis pengencer. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.401-408). Bandung: Fakultas Peternakan Universitas Padjadjaran.
Sopiyana, S., Iskandar, S., Susanti, T. dan Yogaswara, D. 2006. Pengaruh krioprotektan dma, dmf dan glycerol pada proses pembekuan semen ayam kampung. Seminar Nasional Teknologi Peternakan dan Veteriner (pp.702-708). Bogor : Balai Penelitian Ternak.
Toelihere, M. R. 1993. Inseminasi Buatan Pada Ternak. Bandung: Angkasa.
Wirahadikusumah, M. 1985. Biokimia : Metabolism Energy, Karbohidrat and Lipid. Bandung: ITB.
Yudi., Arifiantini, I., Purwantara, B. dan Yusuf, T. L. 2007. Karakteristik semen segar dan kualitas semen cair kuda dalam pengencer dimitropoulos yang disuplementasi dengan fruktosa, trehalosa dan rafinosa. Media Peternakan, 30(3) : 163-172.
Published
2019-09-22
How to Cite
ISNAENI, Mei; FAIDIBAN, O R; TETHOOL, Angelina Novita. Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam). Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), [S.l.], v. 9, n. 2, p. 44 - 49, sep. 2019. ISSN 2620-9403. Available at: <https://journal.fapetunipa.ac.id/index.php/JIPVET/article/view/65>. Date accessed: 12 nov. 2019. doi: https://doi.org/10.30862/jipvet.v9i2.65.