Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review

Bali Cattle Semen Characteristics and Diluent Types: A Review

Main Article Content

Angelina Novita Tethool
Gatot Ciptadi
Sri Wahjuningsih
Trinil Susilawati

Abstract

Abstract

Bali cattle are Indonesian native cattle that are preferred because they have adaptability and reproductive efficiency capabilities.  The productivity of this cow can be increased using artificial insemination.  The success of IB implementation is determined by the quality of the semen used.  This article aims to provide an overview of the quality of fresh semen, after dilution and the types of diluents that have generally been used to maintain Bali cattle spermatozoa.  The quality of fresh semen provides an overview of the condition of the semen after storage. Types of diluents that have been used are chemical diluents such as tris aminomethane, CEP, skim milk, andromed and natural diluents such as coconut water, carrot juice, guava filtrate, honey and tomato juice.  Bali cattle semen stored in each diluent gives a different quality response depending on the diluent used. This diluent has a different response to semen qualities in cold and frozen storage.

Keywords: Quality of semen; Diluent; Bali cattle; Spermatozoa

 

Abstrak 

Sapi Bali merupakan sapi asli Indonesia yang disukai karena memiliki kemampuan adaptasi dan kemampuan efisiensi reproduksi. Produktivitas sapi ini dapat ditingkatkan menggunakan inseminasi buatan. Keberhasilan pelaksanaan IB ditentukan oleh kualitas semen yang digunakan.  Artikel ini bertujuan untuk memberikan gambaran tentang kualitas semen segar, setelah pengenceran dan jenis pengencer yang umumnya telah digunakan untuk mempertahankan spermatozoa sapi Bali.  Kualitas semen segar memberikan gambaran kondisi semen setelah penampungan.  Jenis pengencer yang telah digunakan adalah pengencer dari bahan kimia seperti tris aminomethane, CEP, susu skim, andromed dan pengencer alternatif seperti air kelapa, sari wortel, filtrat jambu biji, madu dan sari buah tomat. Semen sapi Bali yang disimpan pada masing-masing pengencer memberikan respon kualitas yang berbeda-beda tergantung bahan pengencer yang digunakan.  Secara umum pengencer yang digunakan mampu mempertahankan kualitas semen sapi Bali.

Kata kunci: Kualitas semen; Pengencer; Sapi Bali; Spermatozoa

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How to Cite
Tethool, A. N., Ciptadi, G. ., Wahjuningsih, S. ., & Susilawati, T. . (2022). Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review: Bali Cattle Semen Characteristics and Diluent Types: A Review. Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 12(1), 45 –. https://doi.org/10.46549/jipvet.v12i1.214
Section
Articles
Author Biographies

Angelina Novita Tethool, Universitas Papua, Manokwari, Indonesia

Fakultas Peternakan Universitas Papua, Manokwari-Indonesia

Gatot Ciptadi, Universitas Brawijya, Malang-Indonesia.

Fakultas Peternakan Universitas Brawijya, Malang-Indonesia.

Sri Wahjuningsih, Universitas Brawijya, Malang-Indonesia.

Fakultas Peternakan Universitas Brawijya, Malang-Indonesia.

Trinil Susilawati, Universitas Brawijya, Malang-Indonesia.

Fakultas Peternakan Universitas Brawijya, Malang-Indonesia.

References

Aboagla, EME, and Terada T. 2004a. Effects of Egg Yolk during the Freezing Step of Cryopreservation on the Viability of Goat Spermatozoa. Theriogenology. 62(2):1160-117. https://doi.org/10.1016/j.theriogenology.2004.01.013

Aboagla, EME, and Terada T. 2004b. Effects of the Supplementation of Trehalose Extender Containing Egg Yolk with Sodium Dodecyl Sulfate on the Freezability of Goat Spermatozoa. Theriogenology. 62(5):809–818. https://doi.org/10.1016/j.theriogenology.2003.12.003

Ahirwar M, Kataktalware M, Prasad K, Pal R, Barman D, Thul M, and Rawat N. 2018. Effect of Non-Genetic Factors on Semen Quality in Bulls: A review. Journal of Entomology and Zoology Studies. 6(4):38-45.

Aisah S, Isnaini N, dan Wahyuningsih S. 2017. Kualitas Semen Segar dan Recovery Rate Sapi Bali pada Musim yang Berbeda. Jurnal Ilmu-Ilmu Peternakan. 27(1):63-79.

Ariantie OS, Yusuf TL, Sajuthi D, and Arifiantini, RI. 2013. Effect of Glycerol and Dimethylformamide (DMF) Cryoprotectants on Buck Etawah Crossbreed Frozen Semen Using Modified Tris Diluents. Jurnal Ilmu Ternak dan Veteriner, 18(4):239-250. https://doi.org/10.14334/jitv.v18i4.327

Arifiantini RI, dan Yusuf TL. 2006. Keberhasilan Penggunaan Tiga Pengencer dalam Dua Jenis Kemasan pada Proses Pembekuan Semen Sapi Frisien Holstein. Majalah Ilmiah Peternakan. 9(3):1–11. https://doi.org/10.24843/MIP

Astuti ME. 2018. Pengaruh Penambahan Sari Buah Tomat (Solanum lycopersicum) Sebagai Pengencer Alami Terhadap Kualitas Penyimpanan Spermatozoa Sapi Bali (Bos sondaicus). Bionature. 18(2):129-139. https://doi.org/10.35580/bionature.v18i2.6144

Ax RL, Dally M, Didion BA, Lenz RW, Love CC, Varner DD, Hafez B, and Belin ME. 2000. Semen Evaluation, in Reproduction in Farm Animals. Lippincott William & Wilkins: Baltimore, Maryland, USA. 365-375.

Azzahra FY, Setiatin ET, dan Samsudewa D. 2016. Evaluasi Motilitas dan Persentase Hidup Semen Segar Sapi PO Kebumen Pejantan Muda. Jurnal Sain Peternakan Indonesia. 11(2):99–107.

Blegur J, Nalley WM, dan Hine TM. 2020. Pengaruh Penambahan Virgin Coconut Oil dalam Pengencer Tris Kuning Telur terhadap Kualitas Spermatozoa Sapi Bali Selama Preservasi. Jurnal Nukleus Peternakan. 7(2):130–138.

Bohlooli S, Cedden F, PishJang J, Razzaghzadeh S, and Bozoğlu Ş. 2012. The Effect of Different Extenders on Post-thaw Sperm Viability, Motility and Membrane Integrity in Cryopreserved Semen of ZandiRam. Journal of Basic Applied Scientific Research. 2(2):1120–1123.

Cenariu M, Pall E, Borzan M, Bogdan L, and Groza I. 2018. Advanced Techniques of Bovine Semen Analysis. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine. 75(1):58-65. https://doi.org/10.15835/buasvmcn-vm:004317

Deskayanti A, Sardjito T, Sunarso A, Srianto P, Suprayogi TW, and Hermadi HA. 2020. Conception Rate dan Service Per Conception Pada Sapi Bali Hasil Inseminasi Buatan di Kabupaten Sumbawa Barat Tahun 2017. Ovozoa : Journal of Animal Reproduction. 8(2):159-163. https://doi.org/10.20473/ovz.v8i2.2019.

Dey S, Brothag C, and Vijayaraghavan S. 2019. Signaling Enzymes Required for Sperm Maturation and Fertilization in Mammals. Frontiers in Cell and Developmental Biology. 7(341):1–15. https://doi.org/10.3389/fcell.2019.00341

El-Sisy GA, El-Nattat WS, El-Sheshtawy RI, and Abo El-Maaty AM. 2016. Substitution of Egg Yolk with Different Concentrations of Soybean Lecithin in Tris-Based Extender During Bulls' Semen Preservability. Asian Pacific Journal of Reproduction. 5(6):514–518. https://doi.org/10.1016/j.apjr.2016.10.011

Farhana A, Udin Z, Jaswandi J, and Aji RN. 2018. Effect of Extender and Cooling Rate on the Quality of Frozen Thawed Semen of Bali Bull (Bos sondaicus). Buletin Peternakan. 42(4):273-277. https://doi.org/10.21059/buletinpeternak.v42i4.35173

Fazrien WA, Herwijanti E, dan Isnaini N. 2020. Pengaruh Variasi Individu terhadap Kualitas Semen Segar dan Beku Pejantan Unggul Sapi Bali. Sains Peternakan. 18(1):60-65. https://doi.org/10.20961/sainspet.v18i1.37986

Garner DL, and Hafez ESE. 2000. Spermatozoa and Seminal Plasma, in Reproduction in Farm Animals. Lippincott Williams & Wilkins. Baltimore, Maryland, USA. 96- 109.

Hapsari RD, Khalifah Y, Widyas N, Pramono A, dan Prastowo S. 2018. Age Effect on Post Freezing Sperm Viability of Bali Cattle (Bos javanicus). IOP Conference Series: Earth and Environmental Science. 142:1-4. https://doi.org/10.1088/1755-1315/142/1/012007

Haryani R, Toleng AL, Sonjaya H, dan Yusuf M. 2016. Characteristic of Bali Bulls Sperms Assessed using Computerized Assisted Semen Analysis (CASA). International Journal of Sciences: Basic and Applied Research. 28(2):161-168.

Haryanto D, Hartono M, dan Surharyati S. 2015. Beberapa Faktor yang Mempengaruhi Service Per Conception Pada Sapi Bali di Kabupaten Pringsewu. Jurnal Ilmiah Peternakan Terpadu. 3(3):145-150.

Hine TM, Uly K, Nalley WM, dan Armadianto H. 2019. Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide. Jurnal Veteriner. 20(1):93-100. tps://doi.org/10.19087/jveteriner.2019.20.1.93

Hudiatma MD, Prasetiyo D, Haryani E, Pujianto, J, Kuswati, Yekti, APA, and Susilawati, T. 2020. The Success Rate of Artificial Insemination Using Liquid Semen with Different Diluents in Bali Cattle. Russian Journal of Agricultural and Socio-Economic Sciences. 4(100):45-60. https://doi.org/10.18551/rjoas.2020-04.07

Iswanto N, Suyadi, dan Rahmawati A. 2012. Pengaruh α-tocopherol yang Berbeda dalam Pengencer Dasar Tris Aminomethane-Kuning Telur Terhadap Kualitas Semen Kambing Boer Yang Disimpan Pada Suhu 50C. Jurnal Ilmu-Ilmu Peternakan. 22(3):1–8.

Kanchan, and Matharoo JS. 2015. Effect of Semen Colour on Seminal Characteristics in Cattle Bulls. Indian Journal of Animal Research. 49(1):1-46. https://doi.org/10.5958/0976-0555.2015.00031.X

Kulaksiz R, Çebi Ç, Akçay E, and Daşkin A. 2010. The Protective Effect of Egg Yolk from Different Avian Species during the Cryopreservation of Karayaka Ram Semen. Small Ruminant Research. 88(1):12-15. https://doi.org/10.1016/j.smallrumres.2009.11.014

Malik A, Fauzi R, Zakir MI, dan Sakiman S. 2018. Subtitusi Madu Asli Pengganti Gliserol dalam Pembekuan pada Kualitas Pasca-thawing Spermatozoa Sapi Bali. Acta Veterianria Indonesiana. 5(2):98-104. https://doi.org/10.29244/avi.5.2.98-104

Marawali Al, Abdullah MS, dan Jalaludin J. 2019. Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer Air Kelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali. Jurnal Veteriner. 20(1):20-29. https://doi.org/10.19087/jveteriner.2019.20.1.20

Mardiansyah Yuliani E, dan Prasetyo S. 2016. Respon Tingkah Laku Birahi, Service Per Conception, Non Return Rate, Conception Rate pada Sapi Bali Dara dan Induk yang Disinkronisasi Birahi dengan Hormon Progesteron. Jurnal Ilmu dan Teknologi Peteternakan Indonesia. 2(1):134-143.

Martojo H. 2012. Indigenous Bali Cattle is Most Suitable for Sustainable Small Farming in Indonesia. Reproduction in Domestic Animals. 47(suppl.1):10-14. https://doi.org/10.1111/j.1439-0531.2011.01958.x

Mee JF. 2007. The Role of the Veterinarian in Bovine Fertility Management on Modern Dairy Farms. Theriogenology. 68S:S257-S265. https://doi.org/10.1016/j.theriogenology.2007.04.030

Moradpour F. 2019. A Review on Animals Semen Characteristics: Fertility, Reproduction and Development. Asian Journal of Advances in Agricultural Research. 10(2):1–9. https://doi.org/10.9734/ajaar/2019/v10i230024

Nabilla A, Arifiantini RI, dan Purwantara B. 2018. Kualitas Semen Segar Sapi Bali Umur Produktif dan Non-produktif serta Penentuan Konsentrasi Krioprotektan dalam Pengencer Tris Kuning Telur. Jurnal Veteriner. 19(2):242. https://doi.org/10.19087/jveteriner.2018.19.2.242

Nirwana N, and Suparman S. 2017. The Effect of Male Age on the Quality of Bali Cattle Fresh Semen. Chalaza Journal of Animal Husbandry. 2(2):13–1. https://doi.org/10.31327/chalaza.v2i2.296

Nugraha CD, Herwijanti E, Novianti I, Furqon A, Septian WA, Busono W, and Suyadi. 2019. Correlations Between Age of Bali Bull and Semen Production at National Artificial Insemination Center, Singosari - Indonesia. Journal of the Indonesian Tropical Animal Agriculture. 44(3):258-265. https://doi.org/10.14710/jitaa.44.3.258-265

Parera F, Prihatiny Z, Souhoka DF, dan Rizal DM. 2009. Pemanfaatan Sari Wortel Sebagai Pengencer Alternatif Spermatozoa Epididimis Sapi Bali. Journal of the Indonesia Tropical Animinaml Agriculture. 34(1):50-56.

Perumal P. 2014. Scrotal Circumference and Its Relationship with Testicular Growth, Age, and Body Weight in Tho Tho (Bos indicus) Bulls. International Scholarly Research Notices. Article ID 249537. https://doi.org/10.1155/2014/249537

Prastowo S, Dharmawan P, Nugroho T, Bachtiar A, Lutojo, and Pramono A. 2018. Kualitas Semen Segar Sapi Bali (Bos javanicus) pada Kelompok Umur yang Berbeda. Jurnal Ilmu Ternak. 18(1):1-7. https://doi.org/10.24198/jit.v18i1.17684

Purwantara B, Noor RR, Andersson G, and Rodriguez-Martinez H. 2012. Banteng and Bali Cattle in Indonesia: Status and Forecasts. Reproduction in Domestic Animals. 47 suppl 1(s1):2-6. https://doi.org/10.1111/j.1439-0531.2011.4701956.x

Purwoistri R, Susilawati T, dan Rahayu S. 2013. Membran Spermatozoa Hasil Seksing Gradien Albumin Berpengencer Andromed dan Cauda Epididymal Plasma Ditambahkan Kuning Telur. Jurnal Veteriner. 14(3):371–378.

Rahayu S. 2014. The Reproductive Performance of Bali Cattle and It’s Genetic Variation. Berkala Penelitian Hayati. 20(1):28–35. https://doi.org/10.23869/bphjbr.20.1.20145

Raheja N, Choudhary S, Grewal S, Sharma N, and Kumar N. 2018. A Review on Semen Extenders and Additives Used in Cattle and Buffalo Bull Semen Preservation. Journal of Entomology and Zoology Studies. 6(3):239-245.

Rajak SK, Kumaresan A, Gaurav MK, Layek SS, Mohanty TK, Aslam MKM, Tripathi UK, Prasad S, and De S. 2014. Testicular Cell Indices and Peripheral Blood Testosterone Concentrations in Relation to Age and Semen Quality in Crossbred (Holstein Friesian x Tharparkar) Bulls. Asian-Australasian Journal of Animal Sciences. 27(11):1554-1561. https://doi.org/10.5713/ajas.2014.14139

Ratnawati D, Isnaini N, and Susilawati T. 2018. Character Motility of Liquid Semen on Ongole Crossbreed (PO), Bali and Madura Bulls with Diluent CEP-2at Cold Storage. Asian Journal of Microbiology, Biotechnology and Environmental Sciences. 20(1):21–28.

Rosary AR, Kuswati K, dan Susilawati T. 2018. Kualitas Semen Cair Sapi Peranakan Ongole Menggunakan Pengencer CEP-3 Kuning Telur pada Media Simpan yang Berbeda. Jurnal Ternak Tropika. 19(2):87-94. https://doi.org/10.21776/ub.jtapro.2018.019.02.3

Samberi K, Ngadiyono YN, dan Sumadi. 2010. Estimasi Dinamika Populasi dan Produktivitas Sapi Bali di Kabupaten Kepulauan Yapen, Propinsi Papua. Buletin Peternakan. 34(3):169-177.

Sarastina, Susilawati T, dan Ciptadi G. 2007. Analisa Beberapa Parameter Motilitas Spermatozoa Pada Berbagai Bangsa Sapi Menggunakan Computer Assisted Semen Analysis (CASA). Jurnal Ternak Tropika. 6(2):1–12.

Sarsaifi K, Rosnina Y, Ariff M, Wahid H, Hani H, Yimer N, Vejayan J, Win NS, and Abas M. 2013. Effect of Semen Collection Methods on the Quality of Pre- and Post-Thawed Bali Cattle (Bos javanicus) Spermatozoa. Reproduction in Domestic Animals. 48(6):1006-1012. https://doi.org/10.1111/rda.12206

Savitri F, Suharyati S, and Siswanto S. 2014. Kualitas Semen Beku Sapi Bali dengan Penambahan Berbagai Dosis Vitamin C pada Bahan Pengencer Skim Kuning Telur. Jurnal Ilmiah Peternakan Terpadu. 2(3):30-36. https://doi.org/10.23960/jipt.v2i3.499

Setiawan B, Nurul I, and Setyawan IA. 2020. Influence of Bull Age on Fresh Semen Traits of Bali Cattle. Russian Journal of Agricultural and Socio-Economic Sciences. 2(98):27-30. https://doi.org/10.18551/rjoas.2020-02.04

Suhardi R, Megawati N, Ardhani F, Summpunn P, and Wuthisuthimethavee S. 2020. Motility, Viability, and Abnormality of the Spermatozoa of Bali Bull with Andromed® and Egg Yolk-Tris Diluents Stored at 4°C. Iranian Journal of Applied Animal Science. 10(2):249-256.

Sukmawati E, Arifiantini RI, and Purwantara B. 2015. Freezing Capacity of Sperm on Various Type of Superior Bulls. Jurnal Ilmu Ternak dan Veteriner. 19(3):168–175. https://doi.org/10.14334/jitv.v19i3.1079

Sumadiasa IL, Susilawati T, Ciptadi G, and Isnaini N. 2015. The Potency of Guava Filtrate (Psidium guajava linn) for Preservation of Bali Bull Spermatozoa. IOSR-Journal of Agriculture and Veterinary Science. 8(5):51-57.

Sunarti S, Saili T, and Nafiu LO. 2016. Karakteristik Spermatozoa Sapi Bali Setelah Sexing Menggunakan Metode Kolom Albumin dengan Lama Waktu Sexing yang Berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis. 3(1):65-76. https://doi.org/10.33772/jitro.v3i1.1071

Suryanto E, Bulkaini, Ashari, and Karda IW. 2014. Carcass Quality, Marbling and Cholesterol Content of Male Bali Cattle Fed Fermented Cocoa Shell. Journal of the Indonesian Tropical Animal Agriculture. 39(4):249-255. https://doi.org/10.14710/jitaa.39.4.249-255

Susilawati T. 2011. Tingkat Keberhasilan Inseminasi Buatan dengan Kualitas dan Deposisi Semen Yang Berbeda pada Sapi Peranakan Ongole. Jurnal Ternak Tropika. 12(2):15-24.

Susilawati T. 2013. Pedoman Inseminasi Buatan Pada Ternak (Cetakan Pertama). Universitas Brawijaya Press. Malang. 29-120.

Susilawati T, dan Yekti APA. 2018. Teknologi Inseminasi Buatan Menggunakan Semen Cair (Liquid Semen) (Cetakan Pertama). Universitas Brawijaya Press. Malang. 36-116.

Sutarno, and Setyawan AD. 2016. The Diversity of Local Cattle in Indonesia and the Efforts to Develop Superior Indigenous Cattle Breeds. Biodiversitas. 17(1):275-295. https://doi.org/10.13057/biodiv/d170139

Syarifuddin A, Laksmi DNDI, dan Bebas W. 2012. Efektifitas Penambahan berbagai Konsentrasi Glutathion terhadap Daya Hidup dan Motilitas Spermatozoa sapi Bali Post Thawing. Indonesia Medicus Veterinus. 1(2):173-185.

Tarig AA, Wahid H, Rosnina Y, Yimer N, Goh YM, Baiee FH, Khumran AM, Salman H, and Ebrahimi M. 2017. Effect of Different Concentrations of Egg Yolk and Virgin Coconut Oil in Tris-Based Extenders on Chilled and Frozen-Thawed Bull Semen. Animal Reproduction Science. 182:21-27. https://doi.org/10.1016/j.anireprosci.2017.03.024

Vásquez L, Vera O, and Arango J. 2003. Testicular Growth and Semen Quality in Peripuberal Brahman Bulls. Livestock Research for Rural Development. 15(10):76.

Verberckmoes S, Van Soom A, Dewulf J, De Pauw I, and De Kruif A. 2004. Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma. Reproduction in Domestic Animals. 39(6):410-416. https://doi.org/10.1111/j.1439-0531.2004.00521.x

Verberckmoes S, Van Soom A, Dewulf J, and De Kruif A. 2005. Comparison of Three Diluents for the Storage of Fresh Bovine Semen. Theriogenology. 63(3):912-922. https://doi.org/10.1016/j.theriogenology.2004.05.011

Vishwanath R, and Shannon P. 2000. Storage of Bovine Semen in Liquid and Frozen State. Animal Reproduction Science. 62:23-53. https://doi.org/10.1016/S0378-4320(00)00153-6

Widjaya N. 2011. Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5oC. Sains Peternakan. 9(2):72-76. https://doi.org/10.20961/sainspet.v9i2.4796

Yendraliza, Harahap AE, Handoko J, Rodiallah M, and Arman C. 2020. Quality of Bali Bull Cryopreserved Sperm Using Different Extenders And Equilibration Times On Pregnancy Rate Of Bali Cows. Songklanakarin Journal of Science and Technology. 42(3):652-659.

Yendraliza, Tania T, Misrianti R, and Zumarni Z. 2019a. Livability and Recovery Rate of Bali Cattle Spermatozoa during Preservation in Tris-Based Egg Yolk Diluent With Different Sucrose Levels. Jurnal Kedokteran Hewan. 13(2):55–60. https://doi.org/10.21157/j.ked.hewan.v13i2.13033

Yendraliza, Musyrifin M, Elviriadi E, Zumarni Z, dan Rodiallah M. 2019b. Viabilitas Spermatozoa Sapi Bali Menggunakan Pengencer Andromed dengan Penambahan Konsentrasi Sari Wortel yang Berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis. 6(2):239-245. https://doi.org/10.33772/jitro.v6i2.5936

Zulkharnaim, Jakaria, and Noor RR. 2010. Identification of Genetic Diversity of Growth Hormone Receptor (GHR|alu I) Gene in Bali Cattle. Media Peternakan. 33(2):81-87. https://doi.org/10.5398/medpet.2010.33.2.81

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