Kesesuaian Uji Antigen Capture Enzyme Linked Immunosorbant Assay dan Nested Multiplex Polymerase Chain Reaction untuk Diagnosis Rutin Bovine Viral Diarrhea
The agreement of the Antigen Capture Enzyme Linked Immunosorbent Assay and Nested Multiplex Polymerase Chain Reaction for Routine Diagnosis of Bovine Viral Diarrhea
DOI:
https://doi.org/10.46549/jipvet.v11i3.162Keywords:
Antigen capture ELISA, Bovine viral diarrhea, Kappa, Multiplex nested PCRAbstract
Abstract
Bovine Viral Diarrhea (BVD) is one of the main causes of impaired productivity and reproduction of cows. Antigen capture Elisa (ACE) is one of the serological technique that is sensitive, reliable and used regularly for detecting persistent BVD infection individually which simpler than multiplex nested PCR. The aim of this study was to determine the agreement between ACE and multiplex nested PCR as a routine laboratory diagnostic technique to detect the presence of BVD infection. A total of 128 cow serum samples consisting of 63 positive and 65 negative samples based on ACE were used in this study. The samples were collected from active and passive surveillance in dairy and beef cattle conducted by Balai Besar Veteriner (BBVet) Wates. The serum samples were then tested molecularly using multiplex nested PCR against BVD. The result showed 48 out of 63 BVDV-1 positive samples were found positive BVD antigen whereas 57 of 65 BVDV-1 negative samples were negative using multiplex nested PCR, . The agreement value between the two different assays based on statistic analysis using Kappa method was 0.64 and classified a good one. The result concluded that the ACE BVD assay was equally suitable as routine diagnosis to determine BVD infected cattle in the farm.
Keywords: Antigen capture ELISA; Bovine viral diarrhea; Kappa; Multiplex nested PCR.
Abstrak
Bovine Viral Diarrhea (BVD) merupakan salah satu penyebab gangguan produktivitas dan reproduksi sapi. Antigen capture ELISA (ACE) merupakan salah satu teknik serologis yang sensitif, dapat diandalkan dan digunakan secara teratur untuk mendeteksi infeksi BVD persisten secara individual yang lebih sederhana daripada multiplex nested PCR. Tujuan penelitian ini adalah untuk mengetahui kesesuaian antara uji ACE dan multiplex nested PCR sebagai teknik diagnostik laboratorium rutin untuk mendeteksi adanya infeksi BVD. Sebanyak 128 sampel serum sapi yang terdiri dari 63 sampel positif dan 65 negatif berdasarkan ACE BVDV Antigen Test Kit/Serum Plus (Idexx®) digunakan dalam kajian ini. Sampel serum sapi merupakan koleksi dari surveilans aktif dan pasif pada sapi perah dan potong yang dilakukan Balai Besar Veteriner (BBVet) Wates. Sampel serum kemudian diuji secara molekuler menggunakan multiplex nested PCR terhadap BVD. Hasil penelitian menunjukkan bahwa dengan teknik multiplex nested PCR, 48 dari 63 sampel positif BVDV-1 ditemukan positif untuk antigen BVD sedangkan 57 dari 65 sampel negatif BVDV-1 negatif untuk antigen BVD. Analisis statitik berdasarkan perhitungan metoda Kappa menunjukkan nilai kesesuaian antara dua uji sebesar 0,64 dan tergolong bagus. Hasil penelitian menunjukkan kesimpulan bahwa uji ACE BVD sesuai sebagai diagnosis rutin untuk menentukan ternak yang terinfeksi BVD di peternakan.
Kata kunci: Antigen capture ELISA; Bovine viral diarrhea; Kappa; Multiplex nested PCR.
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References
Ammari, M., McCarthy, F. M., Nanduri, B., and Pinchuk, L. M. 2010. Analysis of Bovine Viral Diarrhea Viruses-infected monocytes: identification of cytopathic and non-cytopathic biotype differences. BMC Bioinformatics, 11(Suppl 6): S9.
Brodersen, B. W. 2014. Bovine viral diarrhea virus infections: manifestations of infection and recent advances in understanding pathogenesis and control. Veterinary Pathology, 51(2): 453–464.
Budiharta, S., dan Suardana, I. W. 2007. Buku Ajar Epidemiologi dan Ekonomi Veteriner. Penerbit Universitas Udayana, p. 146-147.
Chang, L., Qi, Y., Liu, D., Du, Q., Zhao, X., and Tong, D. 2021. Molecular detection and genotyping of bovine viral diarrhea virus in Western China. BMC Veterinary Research 17:66
Collins, M. E., Heaney, J., Thomas, C. J., Brownlie, J. 2009. Infectivity of pestivirus following persistence of acute infection. Veterinary Microbiology, 138(2009): 289–296.
Deng, M., Ji, S., Fei1,W., Raza1,S., He,C., Chen, Y., Chen1,H., and Guo, A. 2015. Prevalence Study and Genetic Typing of Bovine Viral Diarrhea Virus (BVDV) in Four Bovine Species in China. PLoS ONE 10(4): e0121718. doi:10.1371/journal.pone.0121718
Dubovi, E. J. 2013. Biologicals Laboratory diagnosis of bovine viral diarrhea virus. Biologicals, 41 : 8–13.
Edmondson, M. A., Givens, M. D., Walz, P. H., Gard, J. A., Stringfellow, D. A., Carson, R. L. 2007. Comparison of tests for detection of bovine viral diarrhea virus in diagnostic samples. J Vet Diagn Invest 19:376–381.
Gilbert, S., Burton, K. M., Prins, S. E., and Deregt, D. 1999. Typing of Bovine Viral Diarrhea Viruses Directly from Blood of Persistently Infected Cattle by Multiplex PCR Typing of Bovine Viral Diarrhea Viruses Directly from Blood of Persistently Infected Cattle by Multiplex PCR. Journal of Clinical Microbiology, 37(6): 2020–2023.
Hilbe, M., Stalder, H., Peterhans, E., Haessig, M., Nussbaumer, M., Egli, C., Schelp, C., Zlinszky, K., and Ehrensperger, F. 2007. Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. Journal of Veterinary Diagnostic Investigation, 19 : 28–34.Iqbal, M., Poole, E., Goodbourn, S., and McCauley, J. W. 2004. Role for Bovine Viral Diarrhea Virus Erns Glycoprotein in the Control of Activation of Beta Interferon by Double-Stranded RNA. Journal of Virology, 78(1): 136-145.
Irianingsih, S.H., Poermadjaja, B., Wuryastuti, H., and Wasito, R. 2020. Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle. IJBiotech Vol 25 (2) : 120-126.
Irianingsih, S.H., Wuryastuty, H., Wasito, R., Wibawa, H., Fadjar, S.T.R., and Poermadjaja, B. 2019. Genetic analysis of NS5B gene from bovine viral diarrhea virus-infected cattle in Central and East Java, Indonesia. Vet World. 2019 Jul; 12(7): 1108–1115.
Isken, O., Langerwisch, U., Schönherr, R., Lamp, B., Schröder, K., Duden, R., Rümenapf, T. H., and Tautz, N. 2014. Functional characterization of bovine viral diarrhea virus nonstructural protein 5A by reverse genetic analysis and live cell imaging. Journal of Virology, 88(1): 82–98.
Kampa, J., Ståhl, K., Renström, L. H. M., and Alenius, S. 2007. Evaluation of a commercial E rns -capture ELISA for detection of BVDV in routine diagnostic cattle serum samples. Acta Veterinaria Scandinavia, 7(49): 1–7.
King,J., Pohlmann,A., Dziadek,K., Beer, M., and Wernike, K. 2021. Cattle connection: molecular epidemiology of BVDV outbreaks via rapid nanopore whole-genome sequencing of clinical samples. BMC Veterinary Research 17:242.
Klemens O, Dubrau D, and Tautz N. 2015. Characterization of the determinants of NS2-3-independent virion morphogenesis of pestiviruses. Journal of Virology, 89(22):11668 –11680.
Kuhne, S., Schroeder, C., Holmquist, G., Wolf, G., Horner, S., Brem, G, and Ballagi, A. 2005. Detection of bovine viral diarrhoea virus infected cattle- testing tissue samples derived from ear tagging using an Erns capture ELISA. Journal of Veterinary Medicine series B-Infectious Disease and Veterinary Public Health, 52(6): 272-277.
Lanyon, S. R., and Reichel, M. P. 2013. Understanding the Impact and Control of Bovine Viral Diarrhoea in Cattle Populations. Springer Science Reviews, 1(1-2): 85-93.
Mahlum, C. E., Haugerud, S., Shivers, J. L., Rossow, K. D., Goyal, S. M., Collins, J. E., and Faaberg, K. S. 2002. Detection of bovine viral diarrhea virus by TaqMan reverse transcription polymerase chain reaction. Journal of Veterinary Diagnostic Investigation, 14(2): 120–125.
Newcomer, B. W., and Givens, D. 2016. Diagnosis and Control of Viral Diseases of Reproductive Importance Infectious Bovine Rhinotracheitis and Bovine Viral Diarrhea. Veterinary Clinic of North America-Food Animal Practice, 32(2): 425-441.
OIE, 2015, Bovine Viral Diarrhoea, in OIE Terrestrial Manual, pp.:1-22.
Saliki, J. T., Huchzermeier, R. O. Y., and Dubovi, E. J. 2000. Evaluation of a New Sandwich ELISA Kit That Uses Serum for Detection of Cattle Persistently Infected with BVD Virus. Annals of New York Academy of Science, 916(1): 358–363.
Saliki, J.T., and Dubovi, E. J. 2004. Laboratory diagnosis of bovine viral diarrhea virus infections. Veterinary Clinics of North America – Food Animal Practice, 20(1): 69–83.
Santman-Berends, I., Mars, M. H., Duijn, L. Van, and Schaik, G. Van. 2015. Evaluation of the epidemiological and economic consequences of control scenarios for bovine viral diarrhea virus in dairy herds. Journal of Dairy Science, 98(11): 7699-7716.
Sudarisman. 2011. Bovine Viral Diarrhea Pada Sapi di Indonesia Dan Permalahannya. Wartazoa, 21(1): 18–24.
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